FIELD: medicine.
SUBSTANCE: invention relates to immunology and oncology. A method for inducing an immune response to tumour cells in a mammalian body is developed, involving a) detection of differences in gene signatures of tumour and normal cells, including prioritization by level of expression of mutant gene, uniqueness, agretopicity; b) synthesis of a set of DNA templates, each of which contains an open reading frame encoding one of the detected gene signatures or an open reading frame encoding from 2 to 80 detected gene signatures; c) synthesis of a set of mRNA based on the obtained set of DNA templates, wherein the mRNA contains the sequence SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:8 at 5'-end; an open reading frame encoding from 1 to 80 tumour gene signatures; sequence SEQ ID NO: 6 or SEQ ID NO: 7 at 3'-end; d) obtaining lipid nanoparticles containing synthesized mRNA; e) introducing the obtained lipid nanoparticles into the mammalian body.
EFFECT: invention enables the immune system to efficiently detect and destroy tumour cells; invention can be used in therapy of patients with oncological diseases of various aetiologies.
1 cl, 4 dwg, 1 tbl, 8 ex
| Title | Year | Author | Number |
|---|---|---|---|
| IMMUNOBIOLOGICAL AGENT FOR THERAPY OF ONCOLOGICAL DISEASES BASED ON mRNA VECTOR | 2024 |
|
RU2828631C1 |
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| mRNA-BASED VECTOR FOR INCREASED TARGET PROTEIN PRODUCTION IN MAMMALIAN CELLS (VARIANTS) | 2022 |
|
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RU2833225C2 |
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|
RU2816137C1 |
| ISOLATED MODIFIED CAPSID PROTEIN VPI OF ADENO-ASSOCIATED VIRUS SEROTYPE 5 (AAV5), CAPSID AND VECTOR BASED THEREON | 2021 |
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|
RU2817891C1 |
