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2 828 885

(13)

(51)

IPC

C12Q1/68(2006-01-01)

C12N15/41(2006-01-01)

(21) (22)

Application

2023131172, 2023-11-29

(24)

Start date

2023-11-29

(22)

Actual filing date

2023-11-29

(45)

Published

2024-10-21

(72)

Inventor

Itani Tarek MokhamedovichChalapa Vladislav Igorevich

(73)

Holder

Federalnoe Biudzhetnoe Uchrezhdenie Nauki Federalnyi Nauchno-Issledovatelskii Institut Virusnykh Infektsii Federalnoi Sluzhby Po Nadzoru V Sfere Zashchity Prav Potrebitelei I Blagopoluchiia Cheloveka Fniivi Rospotrebnadzora)

METHOD FOR DETECTING ENTEROVIRUS D68 GENETIC MATERIAL BY REAL-TIME POLYMERASE CHAIN REACTION Russian patent published in 2024 - IPC C12Q1/68 C12N15/41

Abstract RU 2828885 C1

FIELD: biotechnology; molecular biology; virology.

SUBSTANCE: what is described is a method of detecting genetic material of enterovirus D68 (EV-D68) by real-time polymerase chain reaction (RT-PCR) using a set of primers and a fluorescent labelled probe (Taqman) of the following composition: forward primer qD68-F1 (5'-ACACTGAACCAGARGAAGC-3'), reverse primers qD68-R1 (5'-AAAGCTGCTCTACTRAGAA-3') and qD68-R2 (5'-AAGGCTGCCCTRCTRAGAA-3'), fluorescent labelled probe (Taqman) qD68-P1 (5'-ROX-ACTCGCACAGTGATAAATCARCAYGG-BHQ2-3').

EFFECT: method enables detecting the genetic material EV-D68 by amplifying the VP1 gene fragment coding the surface protein EV-D68 by real-time polymerase chain reaction.

1 cl, 3 dwg, 1 tbl, 1 ex

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RU 2 828 885 C1

Authors

Itani Tarek Mokhamedovich

Chalapa Vladislav Igorevich

Dates

2024-10-21—Published

2023-11-29—Filed