METHOD FOR DETECTION OF INTESTINAL VIRUSES IN CLINICAL SAMPLES OF REAL-TIME MULTIPLEX PCR AND LIST OF SEQUENCIES FOR IMPLEMENTING IT Russian patent published in 2014 - IPC C12Q1/68 

Abstract RU 2506317 C2

FIELD: medicine.

SUBSTANCE: invention refers to laboratory diagnostics, medical virology, molecular biology and epidemiology. The invention aims at detecting and identifying the eleven groups of intestinal viruses (enteroviruses (EV), polioviruses (PV), rotaviruses A and C (RVA and RVB respectively), adenoviruses (ADV), noroviruses (NV), sapoviruses (SV), hepatitis A and E viruses (HAV and HEV, respectively), astroviruses (AV), ortorheoviruses (ORV) in clinical samples and eluates prepared by water concentration in the presence of internal positive control (IPC) by real-time multiplex PCR.

EFFECT: approach enables improving the PCR procedure for the purpose of diagnosing acute intestinal infections (AIIs) and can find application in preventative disinfection departments for the purpose of monitoring of the epidemiologic situation and fast interpretation of the AII breakouts.

7 cl, 4 tbl, 6 ex

Similar patents RU2506317C2

Title Year Author Number
DIFFERENTIAL DIAGNOSTIC TECHNIQUE FOR RESPIRATORY VIRAL INFECTIONS BY REAL-TIME MULTIPLEX PCR AND SEQUENCE LIST FOR IMPLEMENTATION THEREOF 2010
  • Fajzuloev Evgenij Bakhtierovich
  • Nikonova Aleksandra Aleksandrovna
  • Oksanich Aleksej Sergeevich
  • Lobodanov Sergej Aleksandrovich
  • Malakho Sof'Ja Garifovna
  • Zverev Vitalij Vasil'Evich
RU2460803C2
SET OF OLIGODEOXYRIBONUCLEOTIDE PRIMERS AND FLUORESCENT-MARKED DNA PROBES FOR IDENTIFICATION OF RNA ENTEROVIRUSES, ROTOVIRUSES, HEPATITIS A AND E VIRUSES, ADENOVIRUSES, NOROVIRUSES AND ASTROVIRUSES FROM AN AQUEOUS MEDIUM BY MULTIPLEX POLYMERASE CHAIN REACTION 2015
  • Ternovoj Vladimir Aleksandrovich
  • Demina Anna Vladimirovna
  • Chausov Evgenij Vladimirovich
  • Bondarenko Tatyana Yurevna
  • Chub Elena Vladimirovna
RU2610434C1
KIT OF REAGENTS FOR DETECTION AND IDENTIFICATION OF DNA OF BRUCELLOSIS AGENTS BY REAL-TIME POLYMERASE CHAIN REACTION OM-SCREEN-BRUCELLOSIS-RV 2018
  • Sojbanov Vladimir Dmitrievich
  • Sochivko Dmitrij Garrievich
  • Kuznetsovskij Andrej Vladimirovich
  • Vorobev Aleksej Anatolevich
  • Alekseev Yakov Igorevich
  • Onuchina Natalya Viktorovna
  • Minakova Natalya Yurevna
  • Tumanov Aleksandr Sergeevich
RU2715333C1
METHOD FOR DETECTING MODIFIED POLIOVIRUS VACCINE TYPE 2 (nOPV2) RNA BY REAL-TIME RT-PCR 2022
  • Antonov Semen Anatolevich
  • Dolgova Anna Sergeevna
  • Gladkikh Anna Sergeevna
  • Shabalina Anna Vyacheslavovna
  • Ivanova Olga Evgenevna
  • Kozlovskaya Lyubov Igorevna
  • Kanaeva Olga Ilinichna
  • Dedkov Vladimir Georgievich
  • Milashenko Ekaterina Nikolaevna
RU2795703C1
SET OF OLIGONUCLEOTIDES AND METHOD OF MULTIPLEX POLYMERASE CHAIN REACTION IN REAL TIME FOR DETECTION OF RNA SARS-COV-2 2021
  • Abdullaev Adkhamzhon Odilovich
  • Makarik Tatyana Viktorovna
  • Sudarikov Andrej Borisovich
  • Fevraleva Irina Serafimovna
  • Glinshchikova Olga Anatolevna
RU2752902C1
SET OF REAGENTS FOR THE DETERMINATION OF RIBONUCLEIC ACIDS IN THE DIAGNOSIS OF LYMPHOPROLIFERATIVE DISEASES 2023
  • Olkhovskii Igor Alekseevich
  • Gorbenko Aleksei Sergeevich
  • Stoliar Marina Aleksandrovna
  • Bakhtina Varvara Ivanovna
RU2802064C1
METHOD FOR ISOLATION OF INTESTINAL VIRUS RNA FOR ANALYSIS BY RT-PCR METHOD 2006
  • Novikova Nadezhda Alekseevna
  • Golitsyna Ljudmila Nikolaevna
  • Epifanova Natalija Vladimirovna
  • Fedorova Oksana Fedorovna
  • Lukovnikova Ljubov' Borisovna
RU2313792C1
SET OF OLIGODEOXYRIBONUCLEOTIDE PRIMERS AND FLUORESCENT-MARKED DNA PROBES FOR IDENTIFICATION OF RNA OF ENTEROVIRUSES, RHINOVIRUSES, VIRUSES OF HEPATITIS A AND E FROM WATER MEDIUM BY MULTIPLEX PCR METHOD 2013
  • Ternovoj Vladimir Aleksandrovich
  • Sergeeva Elena Igorevna
  • Chausov Evgenij Vladimirovich
  • Bondarenko Tat'Jana Jur'Evna
  • Demina Anna Vladimirovna
  • Shikov Andrej Nikolaevich
  • Agafonov Aleksandr Petrovich
RU2542968C2
TEST SYSTEM FOR DETECTING SARS-CoV-2, INFLUENZA VIRUS A, INFLUENZA VIRUS B BY THE METHOD OF ONE-STEP POLYMERASE CHAIN REACTION WITH REVERSE TRANSCRIPTION 2021
  • Musaeva Tamila Dairovna
  • Timofeeva Mariia Maksimovna
  • Elshin Nikita Dmitrievich
  • Fadeev Artem Viktorovich
  • Komissarov Andrei Borisovich
  • Lioznov Dmitrii Anatolevich
RU2761481C1
TEST SYSTEM AND METHOD FOR DETECTING RNA OF CORONAVIRUS SARS-COV-2, VIRUS-AGENT OF CORONAVIRUS DISEASE 2019 COVID-19, BY POLYMERASE CHAIN REACTION IN REAL TIME (EMBODIMENTS) 2020
  • Filippov Mikhail Aleksandrovich
  • Minashkin Mikhail Mikhajlovich
  • Tatarnikova Olga Gennadevna
  • Pozdnyakova Natalya Vyacheslavovna
RU2731390C1

RU 2 506 317 C2

Authors

Marova Anna Aleksandrovna

Nikonova Aleksandra Aleksandrovna

Zverev Vitalij Vasilevich

Egorova Olga Valerevna

Kalinkina Marina Alekseevna

Oksanich Aleksej Sergeevich

Fajzuloev Evgenij Bakhtierovich

Dates

2014-02-10Published

2012-04-17Filed