FIELD: medicine.
SUBSTANCE: invention refers to laboratory diagnostics, medical virology, molecular biology and epidemiology. The invention aims at detecting and identifying the eleven groups of intestinal viruses (enteroviruses (EV), polioviruses (PV), rotaviruses A and C (RVA and RVB respectively), adenoviruses (ADV), noroviruses (NV), sapoviruses (SV), hepatitis A and E viruses (HAV and HEV, respectively), astroviruses (AV), ortorheoviruses (ORV) in clinical samples and eluates prepared by water concentration in the presence of internal positive control (IPC) by real-time multiplex PCR.
EFFECT: approach enables improving the PCR procedure for the purpose of diagnosing acute intestinal infections (AIIs) and can find application in preventative disinfection departments for the purpose of monitoring of the epidemiologic situation and fast interpretation of the AII breakouts.
7 cl, 4 tbl, 6 ex
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