FIELD: biotechnology.
SUBSTANCE: invention constitutes a set of synthetic oligonucleotide primers for obtaining the full nucleotide sequence of the VP1 gene of the Coxsackie A10 enterovirus, containing two pairs of oligonucleotides specific to the VP1 gene of the Coxsackie A10 enterovirus with activity in a polymerase chain reaction, with the following structure: 1) Forward: 5' GCAAGGATACTGATGAAGTGAC 3' Reverse: 5' GTTGCTGTTTGCCATTGAAAGGC 3'; 2) Forward: 5' CGTCCGTACATGCTGCAA 3' Reverse: 5' GCCCAAATTTACCCATATTAGC 3'.
EFFECT: invention allows ensuring optimal and informative phylogenetic analysis of Coxsackie A10 strains.
1 cl
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Authors
Dates
2021-12-16—Published
2020-09-15—Filed