DIFFERENTIAL DIAGNOSTIC TECHNIQUE FOR RESPIRATORY VIRAL INFECTIONS BY REAL-TIME MULTIPLEX PCR AND SEQUENCE LIST FOR IMPLEMENTATION THEREOF Russian patent published in 2012 - IPC C12Q1/68 C12N15/44 C12N7/00 

FIELD: medicine.

SUBSTANCE: what is described is a differential diagnostic technique for respiratory viral infections by multiplex reverse transcription and real-time PCR. The technique provides analysis of each test sample for the presence of nucleic acids of 11 respiratory viruses in 3 reaction mixtures. The technique enables differential detection of nucleic acids of primary ARVI agents - influenza A and B viruses, coronaviruses, type 1, 2, 3, 4 parainfluenza viruses, adenoviruses, respiratory syncytial virus, rhinoviruses and enteroviruses in biological samples. The presence of nucleic acid of any respiratory virus in the test sample is determined by an increasing fluorescent signal of specific dye in one of the reaction mixture.

EFFECT: invention may be used in preventative disinfection departments for the purpose of epidemiologic situation monitoring and fast interpretation of ARVI breakouts.

6 cl, 4 tbl, 6 ex