METHOD FOR DIAGNOSING TOXICANT LOAD INDEX IN ORAL FLUID FOR FURTHER TREATMENT AND PREVENTION OF TOXICELEMENTOSIS IN PATIENT Russian patent published in 2024 - IPC G01N33/84 G01N33/487 G01N30/72 A61C7/00 

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely to dentistry and medical elementology, and can be used to diagnose the effect of a toxicant – metal: nickel (Ni), cadmium (Cd), lead (Pb) on the body of patients with intraoral orthodontic appliances braces from nickel-titanium alloys. In the patient’s oral fluid determined is level of toxicants: Ni, Cd and Pb and their agonist elements: zinc (Zn), iron (Fe), copper (Cu), calcium (Ca), magnesium (Mg) by inductively coupled plasma mass spectrometry (ICP-MS). According to ICP-MS, an individual toxicant load index (ITLI) is determined by formula ITLI = T/e, where T is the level of toxicants Ni, Cd and Pb in the oral fluid; e is the level of its antagonist element: Zn and Fe (Ni); Zn and Cu (Cd); Ca, Mg, Zn and Fe (Pb) in the oral fluid. ITLI is compared with the reference load index of the given toxicant (RLIT) given in table 1 of the description. If the ITLI is less than the RLIT, the absence of the toxicant effect on the patient’s body is diagnosed; the risk of developing hyperelementosis is low. If the ITLI is greater than the RLIT, the exposure to the toxicant is diagnosed.

EFFECT: method provides the possibility of increasing the accuracy of determining the individual toxicant load index in the patient’s oral fluid (ITLI), its comparison with the RLIT, which allows further planning of treatment of hyperelementosis of toxicant and prevention of its manifestations in the form of inflammatory diseases in oral cavity and state of somatic health of patient, due to non-invasive data acquisition by means of registration of microelement composition at nanostructure level with evaluation of its main elements by means of analysis of oral fluid by mass spectrometry with inductively coupled plasma, which are then converted into evaluation indices according to proposed formulas.

1 cl, 3 tbl