USE OF PARTICLES BASED ON CYTOPLASMIC MEMBRANE, LIPOSOMES AND EXOSOMES FOR ANALYZING ACTIVITY OF IMMUNE CELLS Russian patent published in 2024 - IPC G01N33/50 A61K35/17 A61P35/00 

FIELD: biotechnology.

SUBSTANCE: group of inventions relates to immunotherapy. Disclosed is a method for analysing the activity of a therapeutic natural killer cell (NK cell), involving (i) bringing a therapeutic NK cell into contact with an effective amount of a cytoplasmic membrane particle, a liposome or an exosome, (ii) determining the amount of cytokine produced by the therapeutic NK cell from step (i), (iii) comparing the amount of cytokine produced by the therapeutic NK cell from step (i), with the level of cytokine activity required for using the therapeutic NK cell in immunotherapy, thereby showing the effector function of the therapeutic NK cell, and (iv) selecting the active therapeutic NK cell from step (iii) based on the cytokine content detected at step (ii), where the cytoplasmic membrane-based particles or exosomes are purified from feeding cells which stimulate NK cells. Also disclosed is a method of immunotherapy and use of an active therapeutic natural killer cell as an immunotherapeutic agent.

EFFECT: group of inventions enables to assess the effectiveness of therapeutic immune cells.

14 cl, 4 dwg, 1 ex