METHOD FOR ASSESSING THE EFFECTIVENESS OF CHEMOPREVENTIVE ACTIVITY OF RESVERATROL IN EXPERIMENTAL CARCINOGENESIS OF LIVER CANCER Russian patent published in 2020 - IPC G09B23/28 A61K31/05 A61P35/00 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, more specifically to experimental oncology, pharmacology, namely to a method for assessing the effectiveness of chemoprophylactic activity of resveratrol in experimental carcinogenesis of liver cancer in animals. Method involves administering a substance, personalizing a mathematical model, calculating a probability of developing liver cancer. Resveratrol in dose of 100 mg/kg of body weight is administered into animals together with fodder for 6 months, and biochemical blood values are determined by N-nitrosodiethylamine in dose of 100 mg/l of water for 4 months and 6 months after the beginning of the experiment (MDA, SOD, ALT, AST, Osmotic resistance of erythrocyte membranes, GP, CAT, AP, K⁺-Na⁺-ATPases), obtained data are added to the formula: CDLC=-123.353+(1.58×A)-(0.15×B)-(0.55×C)-(3.11×D)-(4.32×E)-(0.88×F)+(13.66×G)+(0.97×H)-(3.96×I)-(54.85×J), where: CDLC – probability of developing liver cancer (%), A – Osmotic resistance of erythrocyte membranes (in% haemolysis), B – MDA (in Mmol/l), C – SOD (in %), D – ALT (in IU/l), E – AST (in IU/l), F – GP (in mcmol/l), G – CAT (in IU/gHb), H – AP (in nmol/s*l), I – K⁺-Na⁺-ATPases (in mcmol pH/ml protein/p), J is introduction of resveratrol: 1 – if administered, 0 – if not administered. Obtained result is used to assess the development of liver cancer with subsequent evaluation of the effectiveness of chemoprophylactic activity of resveratrol: 0 to 30 % – high chemoprophylactic activity; from 30 % to 60 % – average chemoprophylactic activity; 60–100 % low chemoprophylactic activity.

EFFECT: disclosed method enables assessing the effectiveness of chemoprophylactic activity of resveratrol in experimental carcinogenesis of liver cancer and calculating an individual clinical prediction, having high accuracy.

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