CRYOPRESERVED MALE SEMEN THAWING MEDIUM Russian patent published in 2024 - IPC A61D19/02 

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology, namely to the direction of animal reproduction, includes artificial insemination and cryopreservation of sperm. Medium for thawing cryopreserved male sperm includes: NaCl 0.5 g, KCl 0.035 g, MgSO₄ 7H₂O 0.005 g, KH₂PO₄ 0.005 g, CaCl₂ 2H₂O 0.03 g, NaHCO₃ 0.003 g, glucose 0.05 g, sodium pyruvate 0.03 g, sodium lactate 60% 0.4 g, penicillin 10000 IU, streptomycin sulphate 0.005 g, Phenol red 5% – 0.02 ml, Canine Serum albumin (ALB) 0.4 g, Hepes 2 mmol, taurine 0.001 g and distilled water up to 100 ml.

EFFECT: invention allows increasing sperm cell survival and mobility after thawing due to optimal selection of medium components by weight and volume.

1 cl, 2 tbl