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2 772 905

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(51)

IPC

C12N15/09(2006-01-01)

(21) (22)

Application

2021136728, 2021-12-13

(24)

Start date

2021-12-13

(22)

Actual filing date

2021-12-13

(45)

Published

2022-05-26

(72)

Inventor

Viktorov Denis AleksandrovichToropovskii Andrei NikolaevichShcherbakov Dmitrii NikolaevichNikitin Aleksei Georgievich

(73)

Holder

Obshchestvo S Ogranichennoi Otvetstvennostiu Dzhi"/ Ooo Dzhi"

RECOMBINANT PLASMID PVBL-RBDOMIK PROVIDING SYNTHESIS AND SECRETION OF THE RECOMBINANT RECEPTOR-BINDING DOMAIN (RBD) OF THE SARS-COV-2 CORONAVIRUS LINE B.1.1.529 IN MAMMALIAN CELLS. Russian patent published in 2022 - IPC C12N15/09

Abstract RU 2772905 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology. Described is the plasmid pVBL-RBDomik containing the nucleotide sequence encoding the SARS-CoV-2 RBD protein line B.1.1.529 and providing expression of this RBD protein in mammalian cells and further purification by affinity chromatography. At the same time, the resulting RBD protein is intended for the direct production of a vaccine preparation against SARS-CoV-2-line B.1.1.529.

EFFECT: invention can be used to obtain the RBD protein - the recombinant structural domain of the spike glycoprotein of the Sarbecovirus SARS-CoV-2-line B.1.1.529.

1 cl, 1 dwg, 1 tbl, 4 ex

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RU 2 772 905 C1

Authors

Viktorov Denis Aleksandrovich

Toropovskii Andrei Nikolaevich

Shcherbakov Dmitrii Nikolaevich

Nikitin Aleksei Georgievich

Dates

2022-05-26—Published

2021-12-13—Filed