FIELD: biotechnology.
SUBSTANCE: disclosed is a method of preparing pollen grains for scanning electron microscopy, which comprises fixing fresh pollen grains in 2.5% formaldehyde, filtering and washing pollen grains; then a dehydration step is carried out by successive washing with 70%, 90% and 96% ethanol and final dehydration of the samples with hexamethyldisilazane is carried out.
EFFECT: invention provides an image of the ultrastructure of pollen grains with a pronounced exine and aperture structure.
1 cl, 1 tbl, 1 ex
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