FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, in particular to methods for determining the gene polymorphism associated with resistance to porcine diseases, in particular the WUR10000125 DNA marker in the GBP1 gene associated with resistance to porcine reproductive and respiratory syndrome. Technical result of the disclosed invention is achieved in that disclosed is a method for determining polymorphism A→ G at the position 139666819 SSC4 (genome assembly Sscrofa10.2.) of the gene GBP1 of pigs associated with resistance to porcine reproductive and respiratory syndrome comprising the PCR with real-time results detection where the allele G corresponds to the fluorescence curves on the channel FAM and the allele A corresponds to the fluorescence curves on the channel Su5.
EFFECT: invention enables effective determination of the gene polymorphism associated with resistance to porcine diseases.
1 cl, 1 ex, 2 dwg
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Authors
Dates
2021-04-02—Published
2018-12-05—Filed