METHOD FOR DIAGNOSING MODERATE AND PRONOUNCED SEVERITY OF LIVER INFLAMMATION, CORRESPONDING TO A HISTOLOGICAL ACTIVITY INDEX OF 9 POINTS OR MORE, IN PATIENTS WITH CHRONIC LIVER DISEASES Russian patent published in 2025 - IPC G01N33/49 G01N33/68 G01N33/573 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to gastroenterology, and can be used for diagnosing moderate and pronounced severity of liver inflammation, corresponding to a histological activity index of 9 points and more, in patients with chronic liver diseases. Blood is analysed to determine international normalized ratio (INR), content of matrix metalloproteinase-9 (MMP-9), tissue inhibitor of matrix metalloproteinases-1 (TIMP-1), γ-glutamyl transpeptidase (GGT). Presence or absence of moderate or pronounced severity of liver inflammation is calculated by formula p = 1 : (1 + e^-z), where e is the base of the natural logarithm equal to 2.72; z = a +b₁ × X₁ + b₂ × X₂ + b₃ × X₃; a is a constant equal to -9.077; b₁ is coefficient for ratio TIMP-1/MMP-9, equal to -0.590; X₁ is quantitative value of ratio TIMP-1/MMP-9, expressed in standard units; b₂ is coefficient for GGT, equal to 0.015; X₂ is quantitative value of GGT, expressed in units/l; b₃ is coefficient for INR, equal to 7.599; X₃ is a quantitative value of INR, expressed in standard units. If the p value is less than 0.457, the absence of a moderate or pronounced degree of severity of liver inflammation in the patients with chronic liver diseases is stated. If the p value is 0.457 and more, the presence of a moderate or pronounced degree of severity of liver inflammation corresponding to a histological activity index of 9 and more points in the patients with chronic liver diseases is stated.

EFFECT: method provides the possibility of increasing the sensitivity, specificity and accuracy, reducing the labour intensity and cost of diagnosing a moderate and pronounced degree of severity of liver inflammation, corresponding to a histological activity index of 9 points or more, in patients with chronic hepatic diseases, by determining in the blood of INR, serum content of MMP-9, TIMP-1, GGT with further calculation using the declared mathematical apparatus, without recourse to puncture biopsy of the liver.

1 cl, 4 ex