FIELD: biotechnology.
SUBSTANCE: disclosed is a nucleotide for enzymatic production of L-threonine, containing a nucleotide sequence formed as a result of a mutation consisting in the replacement of guanine (G) with thymine (T) in 520th base of the coding sequence of the spoT gene presented in SEQ ID NO: 13, where the sequence of the mutant nucleotide is a sequence presented in SEQ ID NO: 14. Also disclosed are a recombinant protein for enzymatic production of L-threonine, encoded by said nucleotide, a vector containing said nucleotide, a recombinant bacterium containing said nucleotide, their use for enzymatic production of L-threonine, as well as a method of constructing said recombinant bacteria.
EFFECT: invention provides production of L-threonine in high concentration.
10 cl, 6 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| RECOMBINANT STRAIN BASED ON ESCHERICHIA COLI, A METHOD OF ITS CONSTRUCTION AND USE | 2020 |
|
RU2813283C2 |
| RECOMBINANT STRAIN BASED ON ESCHERICHIA COLI AND METHOD OF ITS CONSTRUCTION AND USE | 2020 |
|
RU2813511C2 |
| RECOMBINANT STRAIN FOR PRODUCTION OF L-AMINO ACID, METHOD FOR CONSTRUCTION AND USE THEREOF | 2020 |
|
RU2832588C1 |
| RECOMBINANT STRAIN WITH MODIFIED BBD29_11265 GENE FOR PRODUCING L-GLUTAMIC ACID, AS WELL AS METHOD FOR CONSTRUCTION AND USE THEREOF | 2021 |
|
RU2841248C1 |
| RECOMBINANT STRAIN WITH MODIFIED BBD29_09525 GENE FOR PRODUCING L-GLUTAMIC ACID, AS WELL AS METHOD FOR ITS CONSTRUCTION AND ITS APPLICATION | 2021 |
|
RU2838163C1 |
| STRAIN HAVING HIGH ABILITY TO PRODUCE L-GLUTAMIC ACID, AS WELL AS METHOD FOR CONSTRUCTION AND USE THEREOF | 2021 |
|
RU2841163C2 |
| METHOD OF OBTAINING L-AMINO ACIDS USING BACTERIA OF FAMILY ENTEROBACTERIACEAE | 2007 |
|
RU2364628C2 |
| METHOD FOR OBTAINING L-AMINO ACID USING BACTERIUM OF Enterobacteriaceae FAMILY, WHICH HAS INCREASED EXPRESSION OF GENES OF STAGE OF FORMATION OF FLAGELLA AND CELLULAR MOVABILITY | 2012 |
|
RU2508404C1 |
| METHOD OF CONSTRUCTING RECOMBINANT BACTERIA BELONGING TO GENUS PANTOEA AND METHOD OF L-AMINO ACIDS PRODUCTION WITH APPLICATION OF BACTERIA BELONGING TO GENUS PANTOEA | 2006 |
|
RU2418069C2 |
| METHOD OF L-THREONINE MANUFACTURE USING BACTERIA OF Escherichia GENUS WITH INACTIVATED GENE yahN | 2007 |
|
RU2392322C2 |
