FIELD: pharmaceutics.
SUBSTANCE: invention refers to pharmaceutical industry, namely to a method for preparing an agent having anti-lipase action. Method for producing an agent having anti-lipase action from vegetative greens of cucumber by extraction of plant material, in which ground plant material is mixed with water at ratio of 1:20 and the mixture is subjected to ultrasonic treatment with operating frequency of 35 kHz and generator power of 100 W at 90 °C for 60 minutes twice, mixture is filtered, water extracts are concentrated in vacuum to 1:30 of initial volume, concentrate is applied on polyamide for chromatography in ratio 1:1, additionally dried to powder state at 50 °C for 6 hours, transferring the powder to a column with pure polyamide for chromatography in ratio of 1:5, washing the column with purified water in a volume equal to 10 volumes of pure polyamide, and 60 % ethanol in volume equal to 5 volumes of pure polyamide, for chromatography, after which the ethanol solution is concentrated to dryness in a vacuum and ground.
EFFECT: method described above enables to obtain an agent of vegetative green of cucumber with high anti-lipase action.
1 cl, 3 ex
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Authors
Dates
2025-03-31—Published
2024-03-14—Filed