FIELD: biotechnology; molecular biology; medical microbiology.
SUBSTANCE: what is presented is a set of oligonucleotide primers for loop isothermal amplification (LAMP) reaction. Set of oligonucleotides includes: primer F3 – "Pfr31-F3" having nucleotide sequence SEQ ID NO: 2, primer B3 – "Pfr3-B3" having nucleotide sequence SEQ ID NO: 3, primer FIP – "Pfr3-FIP", having nucleotide sequence SEQ ID NO: 4, primer BIP – "Pfr3-BIP", having nucleotide sequence SEQ ID NO: 5, loop primer – "Pfr3-LB", having nucleotide sequence SEQ ID NO: 6. What is presented is a method for detecting the Plasmodium falciparum malaria agent by loop isothermal amplification. Method uses primers with a nucleotide sequence SEQ ID NO: 2; SEQ ID NO: 3, SEQ ID NO: 4; SEQ ID NO: 5, SEQ ID NO: 6, which direct synthesis and accumulation of fragments of target gene Pfr364 of malarial plasmodium species Plasmodium falciparum, using SD-polymerase enzyme at 63 °C for maximum of 40 minutes, and determination of target gene is carried out by staining products of amplification reaction with intercalating dye SYTO82.
EFFECT: invention enables high-specificity detecting Plasmodium falciparum malaria agent in DNA samples taken from human blood for 1 hour.
2 cl, 5 dwg, 3 tbl, 4 ex
