FIELD: biotechnology.
SUBSTANCE: invention refers to biotechnology, molecular biology and medical microbiology. Described set of oligonucleotide primers includes: FIP primer – "fopFt182-F3", having nucleotide sequence SEQ ID NO: 2, the BIP primer is "fopFt182-B3" having the nucleotide sequence SEQ ID NO: 3, primer F3 – "fopFt182-FIP" having nucleotide sequence SEQ ID NO: 4, primer B3 – "fopFt182-BIP" having nucleotide sequence SEQ ID NO: 5. Invention enables high-specificity detecting the presence of tularemia pathogen – Francisella tularensis for 1 to 2 hours in samples of pure cultures in concentrations of 10–1,000 mc in ml (depending on strains).
EFFECT: what is described is a set of oligonucleotide primers for the loop isothermal amplification reaction (LAMP), which enables to amplify bacteria-specific Francisella tularensis DNA fragments from the target gene having the nucleotide sequence SEQ ID NO: 1.
3 cl, 4 dwg, 1 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| SET OF OLIGONUCLEOTIDE PRIMERS Ft 101 AND A METHOD FOR DETERMINING FRANCISELLA TULARENSIS (VERSIONS) | 2019 |
|
RU2703803C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS Ft 40 AND A METHOD OF DETERMINING BACTERIA FRANCISELLA TULARENSIS (VERSIONS) | 2019 |
|
RU2706570C1 |
| SET OF REAGENTS AND METHOD FOR DETECTING DNA OF CAUSATIVE AGENTS OF PLAGUE, ANTHRAX AND TULAREMIA BY PCR METHOD WITH HYBRIDISATION-FLUORESCENT RECORDING OF RESULTS | 2013 |
|
RU2542395C1 |
| METHOD OF DETECTING A PLAGUE MICROBE AND A SET OF REAGENTS FOR ITS IMPLEMENTATION | 2022 |
|
RU2796820C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENTLY LABELED PROBES AND A METHOD FOR DETECTING BRUCELLOSIS PATHOGEN DNA | 2022 |
|
RU2795987C1 |
| METHOD FOR IDENTIFICATION OF TULAREMIA PATHOGEN SUBSPECIES FRANCISELLA TULARENSIS SUBSP TULARENSIS, FRANCISELLA TULARENSIS SUBSP MEDIASIATICA AND FRANCISELLA TULARENSIS SUBSP HOLARCTICA | 2015 |
|
RU2612137C1 |
| SET OF FRANCISELLA TULARENSIS BACTERIA STRAINS FOR PRODUCTION OF REFERENCE DNA PREPARATION SET, SET OF DNA PREPARATIONS FOR GENETICALLY DIAGNOSTIC RESEARCH | 2010 |
|
RU2443772C1 |
| METHOD FOR DIFFERENTIATION OF TULAREMIA MICROBIAL SUBSPECIES | 2011 |
|
RU2478717C1 |
| METHOD FOR THE DETERMINATION OF FRANCISELLA TULARENSIS SUBSPECIES BY MULTI-PRIMER PCR | 2021 |
|
RU2765495C1 |
| IMMUNOGENIC COMPOSITION BASED ON RECOMBINANT PSEUDO ADENOVIRAL PARTICLES, AS WELL AS BASED ON PROTEIN ANTIGENS AND A METHOD FOR PRODUCING AN IMMUNOGENIC COMPOSITION | 2018 |
|
RU2691302C1 |
