SET OF OLIGONUCLEOTIDE PRIMERS FT 182 AND A METHOD OF DETERMINING BACTERIA FRANCISELLA TULARENSIS (VERSIONS) Russian patent published in 2019 - IPC C12N1/00 C12Q1/689 G01N33/569 

Abstract RU 2706564 C1

FIELD: biotechnology.

SUBSTANCE: invention refers to biotechnology, molecular biology and medical microbiology. Described set of oligonucleotide primers includes: FIP primer – "fopFt182-F3", having nucleotide sequence SEQ ID NO: 2, the BIP primer is "fopFt182-B3" having the nucleotide sequence SEQ ID NO: 3, primer F3 – "fopFt182-FIP" having nucleotide sequence SEQ ID NO: 4, primer B3 – "fopFt182-BIP" having nucleotide sequence SEQ ID NO: 5. Invention enables high-specificity detecting the presence of tularemia pathogen – Francisella tularensis for 1 to 2 hours in samples of pure cultures in concentrations of 10–1,000 mc in ml (depending on strains).

EFFECT: what is described is a set of oligonucleotide primers for the loop isothermal amplification reaction (LAMP), which enables to amplify bacteria-specific Francisella tularensis DNA fragments from the target gene having the nucleotide sequence SEQ ID NO: 1.

3 cl, 4 dwg, 1 tbl, 3 ex

Similar patents RU2706564C1

Title Year Author Number
SET OF OLIGONUCLEOTIDE PRIMERS Ft 101 AND A METHOD FOR DETERMINING FRANCISELLA TULARENSIS (VERSIONS) 2019
  • Shchit Irina Yurevna
  • Biketov Sergej Fedorovich
  • Dyatlov Ivan Alekseevich
RU2703803C1
SET OF OLIGONUCLEOTIDE PRIMERS Ft 40 AND A METHOD OF DETERMINING BACTERIA FRANCISELLA TULARENSIS (VERSIONS) 2019
  • Shchit Irina Yurevna
  • Biketov Sergej Fedorovich
RU2706570C1
SET OF REAGENTS AND METHOD FOR DETECTING DNA OF CAUSATIVE AGENTS OF PLAGUE, ANTHRAX AND TULAREMIA BY PCR METHOD WITH HYBRIDISATION-FLUORESCENT RECORDING OF RESULTS 2013
  • Mokrievich Aleksandr Nikolaevich
  • Kudrjavtseva Tamara Jur'Evna
  • Varlamov Dmitrij Aleksandrovich
  • Sochivko Dmitrij Garrievich
  • Djatlov Ivan Alekseevich
RU2542395C1
METHOD OF DETECTING A PLAGUE MICROBE AND A SET OF REAGENTS FOR ITS IMPLEMENTATION 2022
  • Shchit Irina Iurevna
  • Sheviakov Anton Georgievich
  • Vetchinin Sergei Sergeevich
  • Biketov Sergei Fedorovich
  • Diatlov Ivan Alekseevich
RU2796820C1
SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENTLY LABELED PROBES AND A METHOD FOR DETECTING BRUCELLOSIS PATHOGEN DNA 2022
  • Shchit Irina Iurevna
  • Biketov Sergei Fedorovich
RU2795987C1
METHOD FOR IDENTIFICATION OF TULAREMIA PATHOGEN SUBSPECIES FRANCISELLA TULARENSIS SUBSP TULARENSIS, FRANCISELLA TULARENSIS SUBSP MEDIASIATICA AND FRANCISELLA TULARENSIS SUBSP HOLARCTICA 2015
RU2612137C1
SET OF FRANCISELLA TULARENSIS BACTERIA STRAINS FOR PRODUCTION OF REFERENCE DNA PREPARATION SET, SET OF DNA PREPARATIONS FOR GENETICALLY DIAGNOSTIC RESEARCH 2010
  • Osina Natal'Ja Aleksandrovna
  • Utkin Denis Valer'Evich
  • Senichkina Ajslu Mukhamjatovna
  • Bugorkova Tat'Jana Vasil'Evna
  • Kutyrev Vladimir Viktorovich
RU2443772C1
METHOD FOR DIFFERENTIATION OF TULAREMIA MICROBIAL SUBSPECIES 2011
  • Vakhrameeva Galina Mikhajlovna
  • Lapin Aleksandr Aleksandrovich
  • Timofeev Vitalij Sergeevich
  • Mokrievich Aleksandr Nikolaevich
  • Kudrjavtseva Tamara Jur'Evna
  • Pavlov Vitalij Mikhajlovich
RU2478717C1
METHOD FOR THE DETERMINATION OF FRANCISELLA TULARENSIS SUBSPECIES BY MULTI-PRIMER PCR 2021
  • Sorokin Vladimir Mikhajlovich
  • Vodopyanov Aleksej Sergeevich
  • Pavlovich Natalya Vladimirovna
  • Tsimbalistova Marina Vladimirovna
RU2765495C1
IMMUNOGENIC COMPOSITION BASED ON RECOMBINANT PSEUDO ADENOVIRAL PARTICLES, AS WELL AS BASED ON PROTEIN ANTIGENS AND A METHOD FOR PRODUCING AN IMMUNOGENIC COMPOSITION 2018
  • Ershova Anna Stepanovna
  • Boksha Irina Sergeevna
  • Lavrova Natalya Vitalevna
  • Lyashchuk Aleksandr Mikhajlovich
  • Gromov Aleksandr Viktorovich
  • Poponova Mariya Sergeevna
  • Sherbinin Dmitrij Nikolaevich
  • Alekseeva Svetlana Viktorovna
  • Viskova Natalya Yurevna
  • Kalugina Irina Alekseevna
  • Dovzhenko Nina Aleksandrovna
  • Kostarnoj Aleksej Viktorovich
  • Gancheva Petya Gancheva
  • Shmarov Maksim Mikhajlovich
  • Lunin Vladimir Glebovich
  • Karyagina-Zhulina Anna Stanislavovna
RU2691302C1

RU 2 706 564 C1

Authors

Shchit Irina Yurevna

Biketov Sergej Fedorovich

Kulikalova Elena Stanislavovna

Dates

2019-11-19Published

2019-02-14Filed