FIELD: biotechnology.
SUBSTANCE: invention relates to a set of oligonucleotide primers and a DARQ probe are proposed, which allow to detect a target gene DNA fragments specific for pathogenic Brucella. To detect the DNA of pathogenic Brucella, a combined PCR-LAMP reaction with real-time product detection is carried out.
EFFECT: claimed invention allows for 20 minutes with high specificity to detect the presence of the causative agent of brucellosis in samples of pure cultures at a concentration of 1×101 mc/ml.
2 cl, 3 dwg, 2 tbl, 3 ex
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