FIELD: biotechnology.
SUBSTANCE: invention relates to molecular genetics and biotechnology. Disclosed is a method of creating a recombinant strain of enterococcus L3-SARS"XBB.1.5" by electroporation of a culture of enterococci Enterococcus faecium L3 of recombinant plasmid DNA pentF-sars"XBB.1.5", obtained from plasmid DNA pentF-pspf, in which the pneumococcus pspf gene insert is removed and replaced with the sars"XBB.1.5" gene insert. Recombinant plasmid pentF-sars"XBB.1.5"is also provided.
EFFECT: inventions can be used to produce a vaccine against coronavirus infection.
2 cl, 4 dwg, 1 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR CREATING RECOMBINANT ENTEROCOCCUS L3-SARSN1 STRAIN BASED ON BIOLOGICALLY ACTIVE ENTEROCOCCUS FAECIUM L3 STRAIN | 2022 |
|
RU2820058C1 |
| METHOD FOR CREATING LIVE STRAIN OF ENTEROCOCCUS L3-SARS BASED ON BIOLOGICALLY ACTIVE STRAIN OF E. FAECIUM L3 | 2021 |
|
RU2782529C1 |
| METHOD OF CREATING A LIVE VACCINE AGAINST COVID-19 BASED ON THE PROBIOTIC STRAIN ENTEROCOCCUS FAECIUM L3 AND A LIVE VACCINE ENTEROCOCCUS FAECIUM L3-PENTF-COVID-19 | 2020 |
|
RU2745626C1 |
| LIVE VACCINE BASED ON ENTEROCOCCUS FAECIUM L3 PROBIOTIC STRAIN FOR PREVENTION OF INFECTION CAUSED BY STREPTOCOCCUS PNEUMONIE | 2018 |
|
RU2701733C1 |
| METHOD FOR INTROGRESSION OF PATHOGENIC STREPTOCOCCUS GENES IN CHROMOSOMAL DNA OF PROBIOTIC STRAIN OF ENTEROCOCCUS FAECIUM L3 FOR EXPRESSION IN PILES | 2015 |
|
RU2640250C2 |
| RECOMBINANT DNA ENSURING PRODUCTION OF THE RECOMBINANT PROTEIN COV1 EXHIBITING IMMUNOGENIC PROPERTIES AGAINST SARS-COV-2 VIRUS | 2021 |
|
RU2776484C1 |
| RECOMBINANT PLASMID pVEAL3-XBB.1.5, WHICH PROVIDES SYNTHESIS AND SECRETION OF RECEPTOR-BINDING DOMAIN RBD S OF SARS-CoV-2 PROTEIN OF VARIANT XBB.1.5, AND RECOMBINANT STRAIN CHO-XBB.1.5 - PRODUCER OF RECEPTOR-BINDING DOMAIN RBD S PROTEIN SARS-CoV-2 VERSION XBB.1.5 INTENDED FOR PRODUCING IMMUNOBIOLOGICAL PREPARATIONS | 2024 |
|
RU2839373C1 |
| INTEGRATIVE PLASMID VECTOR pVEAL3-RBDdel, PROVIDING SYNTHESIS AND SECRETION OF RECOMBINANT PROTEIN OF RECEPTOR-BINDING DOMAIN RBDdelta OF CORONAVIRUS SARS-CoV-2 IN MAMMALIAN CELLS, RECOMBINANT CELL LINE STRAIN CHO-K1-RBDdelta AND RECOMBINANT PROTEIN RBDdelta SARS-CoV-2 PRODUCED BY CELL LINE STRAIN | 2023 |
|
RU2816175C1 |
| ARTIFICIAL GENETIC CONSTRUCT FOR THE HETEROLOGOUS EXPRESSION OF THE S-PROTEIN RECEPTOR-BINDING DOMAIN IN A FUSED POLYPEPTIDE CHAIN WITH A NUCLEOCAPSID PROTEIN | 2022 |
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RU2801597C1 |
| RECOMBINANT VACCINE STRAIN FOR LIVE INTRANASAL VACCINE PROVIDING COMBINED PREVENTION OF INFLUENZA AND CORONAVIRUS INFECTIONS | 2022 |
|
RU2782531C1 |
