METHOD OF SAMPLING BIOLOGICAL MATERIAL FOR MICROBIOLOGICAL ANALYSIS USING A STERILE BRUSH Russian patent published in 2025 - IPC A61M1/00 A61C19/00 

FIELD: medical science.

SUBSTANCE: invention refers to medicine, namely to dentistry, and is applicable in microbiological analysis of a biological material. Graduated probe is used to determine a periodontal pocket depth within the examined tooth. Based on the obtained result, a brush of the corresponding size and shape is selected from the set of brushes. Selected brush is then sterilized in an autoclave. After sterilization, without touching the bristles, the pocket content is sampled by introducing the brush into the gingival pocket in parallel to the tooth axis with visually controlling the immersion depth, making 2 clockwise rotations of the brush around its axis and 2 counter-clockwise rotations around its axis. Thereafter, the brush is removed from the periodontal pocket and placed into a sterile test tube for further microbiological examination. Pre-determined size and shape of the brush are used when performing daily hygienic procedures at home. Used brushes can have a cylindrical or conical shape, different bristle hardness.

EFFECT: by maintaining the integrity of the mucosa during the procedure, as well as further use of the interdental brush for hygienic measures at home, the method enables more accurate diagnosis and prescribing the adequate and well-timed treatment.

7 cl, 2 ex