METHOD OF PREPARING OF NUTRIENT MEDIUM FOR BIFIDOBACTERIUM LABORATORY FERMENTING AGENT PRODUCING Russian patent published in 1994 - IPC

FIELD: milk, medicinal and microbiological industries. SUBSTANCE: lactose and magnesium chloride are dissolved preliminary in 1M phosphate buffer solution, then preparation of yeast β-galactosidase is added (3-4 U/ml), and hydrolysis is carried out up to the oligosaccharide formation. Then pepton, corn extract, agar-agar are added to the prepared hydrolysate, and volume of mixture is brought about with water to 1000 ml. Quantity of viable bifidobacterium cells is 10⁹-10¹⁰ ml. EFFECT: accelerated process of primary fermenting agent recovery, increased activity of its. 4 tbl