FIELD: biotechnology, genetic engineering. SUBSTANCE: recombinant plasmid pVH 64 (size is 5804 nucleotide pairs) contains vector part of plasmide pBR 322 and Hind III-P-fragment of DNA of smallpox virus, strain LIVP. Cloning Hind III-P-fragment contains genes of proteins 36k and 12k of smallpox virus, strain LIVP. Vector plasmide contains bla-gene providing resistance to ampicillin. Prepared plasmide is used for mapping of viral genes and as a molecular probe for detection of homologous fragments of any orthopox-viruses. Recombinant plasmide pVH 64 can be used for preparing of bacterial producers of viral proteins, and as a vector for incorporation of foreign information into viral genome. Nucleotide sequence of Hind III-P-fragment is determined. EFFECT: preparing of recombinant plasmid DNA indicated above. 3 cl, 1 tbl
| Title |
Year |
Author |
Number |
| STRAIN OF RECOMBINANT SMALL POX VIRUS EXPRESSING STRUCTURAL PROTEINS OF THE HORSE VENEZUELAN ENCEPHALOMYELITIS VIRUS AND USEFUL FOR IMMUNOBIOLOGICAL PREPARATIONS PRODUCTION AND A METHOD OF ITS PREPARING |
1993 |
- Frolov I.V.
- Urmanov I.Kh.
- Kolykhalov A.A.
- Netesov S.V.
- Agapov E.V.
- Serpinskij O.I.
- Svjatchenko V.A.
|
RU2091489C1 |
| RECOMBINANT PLASMID DNA PC-NS3 PROVIDING INTEGRATION OF COMPLEX OF GENES C, PRM, E, NS1, NS 2A, NS 2B, NS3 OF TICK-BORNE ENCEPHALITIS VIRUS IN GENOME OF BOVINE VACCINE VIRUS AND RECOMBINANT STRAIN OF BOVINE VACCINE VIRUS EXPRESSING COMPLEX OF GENES C, PRM, E, NS1, NS 2A, NS2B, NS3 OF TICK-BORNE ENCEPHALITIS VIRUS IN IMMUNIZED ORGANISM CELLS |
1995 |
- Gajnullina M.N.
- Dmitriev I.P.
- Dobrikova E.Ju.
- Khromykh A.A.
- Ignat'Ev G.M.
- Pugachev K.V.
- Ageenko V.A.
- Vorob'Eva M.S.
- Pletnev A.G.
- Beljaev A.S.
- Sandakhchiev L.S.
|
RU2112038C1 |
| VECTOR PLASMID DNK PTK 1261, INTENDED FOR INTEGRATION OF A STRANGE FRAGMENT INTO GENOME OF VIRUS OF VARIOLOVACCINE AND METHOD FOR PREPARING SAME |
0 |
- Prikhodko Grigorij Grigorevich
- Urmanov Ilnur Khamidovich
- Serpinskij Oleg Igorevich
- Mikryukov Nikolaj Nikolaevich
- Chizhikov Vladimir Evgenevich
|
SU1640163A1 |
| VECTOR PLASMID DNK PTK 1285 INTENDED FOR INTEGRATION OF A FOREIGN FRAGMENT INTO GENOM OF VIRUS OF VARIOLOVACCINE AND METHOD OF PREPARING SAME |
0 |
- Prikhodko Grigorij Grigorevich
- Urmanov Ilnur Khamidovich
- Serpinskij Oleg Igorevich
- Mikryukov Nikolaj Nikolaevich
- Chizhikov Vladimir Evgenevich
|
SU1640164A1 |
| RECOMBINANT PLASMID DNA P28CME FOR OBTAINING VACCINE AGAINST ENCEPHALITIS |
0 |
- Belyaev A.S.
- Khromykh A.A.
- Malygin E.G.
- Pletnev A.G.
- Matveev L.E.
- Shevlyagina L.R.
- Putintseva N.I.
- Danilyuk N.K.
- Vtorushina I.A
- Sinyakov A.N.
- Prikhodko G.G.
- Mamaev L.V.
- Kulichkov V.A.
- Yamshchikov V.F.
- Dobrikova E.Yu.
- Pressman E.K.
|
SU1490963A1 |
| RECOMBINANT PLASMID DNA PVAX2 FOR TRANSFER AND EXPRESSION OF HEPATITIS B SURFACE ANTIGEN GENE IN VARIOLOVACCINE VIRUS GENOME AND A STRAIN OF VARIOLOVACCINE VIRUS EXPRESSING THE SURFACE ANTIGEN OF HEPATITIS B VIRUS |
1985 |
- Al'Tshtejn A.D.
- Andzhaparidze O.G.
- Antonova T.P.
- Baev A.A.
- Bendukidze K.A.
- Gorodetskij S.I.
- Zakharova L.G.
- Krjazhevskaja M.V.
- Pashvykina G.V.
- Fodor I.I.
- Chernos V.I.
|
SU1354709A1 |
| RECOMBINANT PLASMID DNA 36K-PVH, CODING SYNTHESIS OF IMMUNOGENIC PROTEIN 36K OF SMALLPOX VACCINE, STRAIN LIVP, AND STRAIN OF BACTERIA ESCHERICHIA COLI - A PRODUCER OF THIS PROTEIN |
0 |
- Ryazankina Olga Illarionovna
- Muravlev Aleksandr Ivanovich
- Cheshenko Nataliya Viktorovna
- Chikaev Nikolaj Andreevich
- Shchelkunov Sergej Nikolaevich
- Malygin Ernst Georgievich
|
SU1661215A1 |
| RECOMBINANT PLASMID DNA PTBI-HBSAG COMPRISING CHIMERIC GENE TBI-HBSAG UNDER CONTROL OF SMALLPOX VACCINE PROMOTER P7,5K AND STRAIN OF RECOMBINANT SMALLPOX VACCINE INDUCING IMMUNE RESPONSE AGAINST HIV AND HUMAN HEPATITIS B IN ANIMAL BODY |
2001 |
- Shchelkunov S.N.
- Rjazankin I.A.
- Ageenko V.A.
- Ignat'Ev G.M.
- Babkin I.V.
|
RU2194075C2 |
| RECONBINANT PLASMID DNA P7,5S 11 CME FOR OBTAINING VACCINE AGAINST ENCEPHALITIS AND HAPATITIS |
0 |
- Belyaev A.S.
- Dmitriev I.P.
- Putintseva N.I.
- Shevlyagina L.R.
- Baklanov M.M.
- Ammosov A.D.
- Mironova E.B.
- Pletnev A.G.
- Pressman E.K.
- Matveev L.E.
|
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| RECOMBINANT PLASMID DNA ENCODING SYNTHESIS OF HBS AG HEPATITIS VIRUS B, AND A STRAIN OF VACCINE VIRUS - A PRODUCER OF HBS AG HEPATITIS VIRUS B |
1988 |
- Gibadulin R.A.
- Grodnitskaja N.A.
- Denisova G.F.
- Dzjubenko O.V.
- Dolgushina N.I.
- Lideman L.F.
|
SU1515698A1 |
