METHOD OF CALMODULIN PREPARING Russian patent published in 1994 - IPC

Abstract RU 2015514 C1

FIELD: preparative biochemistry. SUBSTANCE: testicles of cattle animals are defatted with acetone, extracted with acetic acid solution at pH 4.7-4.9 and 18-20 C for 1.5-2 h, precipitate is separated, homogenized with water and salted out with sodium chloride (50-70 g/l). Supernatant is separated, calmodulin is precipitated at pH 4.0-4.3 and purified by dialysis, precipitation by ethyl alcohol and reprecipitation by acetone. EFFECT: improved method of calmodulin preparing. 2 dwg, 1 tbl

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RU 2 015 514 C1

Authors

Stekol'Nikov L.I.

Ryl'Tsev V.V.

Filatov V.N.

Bychkov B.I.

Dates

1994-06-30—Published

1992-02-06—Filed