FIELD: medicine. SUBSTANCE: testicles of cattle are used as a source of animal raw. Thrombin (50-100 U/l) is added to extract and kept for 2-4 h at 37-40 C, pH 5-7. Desalting is carried out by sodium chloride at concentration 50-70 g/l extract, and extract purification is carried out by successive treatment at 0-6 C with 3-4 volumes of acetone, then - with ethanol and hydrochloric acid solution at 4-6 C, pH 4.5-4.7. EFFECT: increased yield and activity of factor XIII in blood coagulation.
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