METHOD OF CYTOCHROME C PREPARING Russian patent published in 1998 - IPC

Abstract RU 2105559 C1

FIELD: biochemistry. SUBSTANCE: method involves salmon heart stuffing extraction with trichloroacetic or sulfuric acid at pH 4.8-5.6, separation of inert proteins on natural adsorbent perlite added to extractive mass at amount 20-30 g/100 g parent raw followed by extract separation, its neutralization to neutral pH value, precipitation of proteins with crystalline ammonium sulfate, cytochrome c precipitation with trichloroacetic acid or sulfuric acid, its washing with ammonium sulfate saturated solution to separate impurities, dissolving in water followed by freezing-thawing, dialysis, sterile filtration and package. The obtained cyrochrome c shows the most similar amino acid composition with that of human cytochrome c. EFFECT: improved method of preparing. 3 cl

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RU 2 105 559 C1

Authors

Ehpshtejn L.M.

Cherednichenko S.I.

Bystrova I.M.

Sidorova N.D.

Selivanov E.A.

Dates

1998-02-27Published

1995-01-17Filed