FIELD: virology. SUBSTANCE: after trypsin treatment kidney cells of Sirian hamster is infected into suspension with fixed rabies virus of strain Vnukovo-32, 32-38 passages. Infected cells at concentration 500-100000 in 1 ml were loaded in bioreactor in which microcarrier Cytolar (concentration is 10 g/l growth medium) exists. Cultivation is carried out at discrete regime of stirring: stirring time is 2-5 min, pause period at the first day is 120 min following by decrease of pause time by 30 min every day; from 4-th day and further pause time is 30 min. Stirring rate is 15-20 rev/min. EFFECT: improved method of vaccine preparing.
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF PREPARING VIRUS-CONTAINING MATERIAL FOR ANTIRABIC VACCINE MAKING | 1994 |
|
RU2083668C1 |
| METHOD OF STERILIZING FILTRATION OF VIRUS-CONTAINING MATERIAL FOR PRODUCING CULTURAL ANTI-RABIC VACCINE | 1989 |
|
RU1755580C |
| METHOD FOR PREPARING HIGHLY SPECIFIC HETEROLOGOUS ANTIRABIES SERUM | 2006 |
|
RU2322503C1 |
| METHOD OF PREPARING VACCINE AGAINST RABIES AND CARNIVOROUS PLAGUE | 1994 |
|
RU2080125C1 |
| METHOD OF PHYLOVIRUS SUSPENSION CULTURING IN CELL CULTURE ON MICROCARRIERS | 1993 |
|
RU2076905C1 |
| METHOD OF PREPARING VACCINE FOR TICK-BORNE ENCEPHALITIS CONTROL | 1993 |
|
RU2082432C1 |
| METHOD OF PRODUCTION OF ANTIRABIC VACCINE | 2013 |
|
RU2538617C2 |
| RABIES VACCINE PRODUCTION METHOD | 1986 |
|
SU1367487A1 |
| METHOD OF PRODUCING CULTURE INACTIVATED RABIES VIRUS VACCINE | 2023 |
|
RU2816765C1 |
| ENA-21/13-13-FINITE MONOLAYER-SUSPENSION CELL SUBLINE OF KIDNEY OF NEW-BORN SYRIAN HAMSTER, INTENDED FOR REPRODUCTION OF FOOT-AND-MOUTH DISEASE VIRUS AND RABIES VIRUS | 2014 |
|
RU2553552C1 |
