FIELD: medicinal industry. SUBSTANCE: method involves the use of transplantable heteroploid cells Vero. To cell suspension at concentration 100000 cells/ml the strain of rabies virus Vnukovo-32 adapted to these cells is added at dose 0.1 LD50/cell. Culturing is carried out in bioreactor of company "Techne" (capacity - 3 l) at addition of microcarrier "Cytolar-2" at concentration 3 mg/ml culture medium. On the 3-d day the culture medium is changed for supporting medium. On the 4-7-th days fresh portion of infected cells is sown additionally in reactor. Cells were collected every day after culture medium change for supporting medium. EFFECT: improved method of preparing. 1 dwg, 1 tbl
Title | Year | Author | Number |
---|---|---|---|
METHOD OF PREPARING CULTURAL ANTIRABIES VACCINE | 1991 |
|
RU2032738C1 |
METHOD FOR PREPARING HIGHLY SPECIFIC HETEROLOGOUS ANTIRABIES SERUM | 2006 |
|
RU2322503C1 |
0 |
|
SU1782990A1 | |
METHOD OF PHYLOVIRUS SUSPENSION CULTURING IN CELL CULTURE ON MICROCARRIERS | 1993 |
|
RU2076905C1 |
METHOD OF PRODUCING FOUR-COMPONENT LIVE CULTURE VACCINE AGAINST MEASLES, VARICELLA, MUMPS, AND RUBELLA | 2019 |
|
RU2717769C1 |
METHOD OF PRODUCING CULTURE INACTIVATED RABIES VIRUS VACCINE | 2023 |
|
RU2816765C1 |
METHOD FOR PREPARING INACTIVATED ANTIRABIC VACCINE IN FRAMELESS CELL GROWTH AND VIRUS REPLICATION IN SHORT-TERM CULTURE CYCLE | 2012 |
|
RU2537183C2 |
ENA-21/13-13-FINITE MONOLAYER-SUSPENSION CELL SUBLINE OF KIDNEY OF NEW-BORN SYRIAN HAMSTER, INTENDED FOR REPRODUCTION OF FOOT-AND-MOUTH DISEASE VIRUS AND RABIES VIRUS | 2014 |
|
RU2553552C1 |
METHOD FOR PREPARING ANTIRABIC VACCINE | 2005 |
|
RU2287343C1 |
BHK-21/SUSP/ARRIAH - CONTINUOUS SUSPENSION SUBLINE OF NEWBORN SYRIAN HAMSTER KIDNEY CELLS, INTENDED FOR REPRODUCTION OF FOOT-AND-MOUTH DISEASE VIRUSES, RABIES, PARAINFLUENZA-3, AUJESZKY'S DISEASE IN PRODUCING ANTIVIRAL VACCINES, AS WELL AS FOR MAKING DIAGNOSTIC AND PREVENTIVE VETERINARY BIOPREPARATIONS | 2019 |
|
RU2722671C1 |
Authors
Dates
1997-07-10—Published
1994-12-07—Filed