METHOD OF PREPARING VACCINE FOR TICK-BORNE ENCEPHALITIS CONTROL Russian patent published in 1997 - IPC

Abstract RU 2082432 C1

FIELD: immunobiotechnology. SUBSTANCE: method involves infection of cell culture with virus followed by its reproduction and inactivation, isolation, purification and sorption on viral antigen adjuvant. Cell culture is immobilized on collagen microcarrier at particle diameter 160-250 mcm and infected with tick-borne encephalitis virus at dose 5-30 LD₅₀/cell. Antigen is isolated by sedimentation treatment at separation factor 7000-12000. For infection expediently to use diploid cells of human fetal lung culture or disaggregated culture of chicken embryo fibroblasts. EFFECT: improved method of vaccine preparing. 3 cl, 4 tbl

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RU 2 082 432 C1

Authors

Kiseleva N.N.

Osipova E.G.

Sokol'Nikova G.A.

Popova N.A.

Mirjutova T.L.

Dates

1997-06-27—Published

1993-07-19—Filed