METHOD FOR PREPARING HIGHLY SPECIFIC HETEROLOGOUS ANTIRABIES SERUM Russian patent published in 2008 - IPC C12P21/00 A61K39/42 C07K16/08 A61P31/12 

FIELD: biotechnology, preparative biochemistry.

SUBSTANCE: method involves hyperimmunization of animal-producers with inactivated, purified and concentrated rabies virus with aluminum phosphate and/or sodium nucleinate as an adjuvant. Virus obtained in culture transplantable cells VERO and in primary culture of Syrian hamster kidney cells is concentrated by adsorption of aluminum phosphate gel or centrifugation, or ultrafiltration methods and purified passing through porous silica, or by ion-exchange chromatography. For immunization antigen with the immunogenicity index 9.5 IU/ml, not less, is used and with the content of protein less 200 mcg/ml, bovine serum albumin less 0.5 mcg/ml Syrian hamster kidney cells and VERO cells less 0.5 mcg/ml, cellular DNA 5.0-10.0 ng/ml, aluminum ions concentration in antigen 0.2-1.25 mcg/ml, and sodium nucleinate 0.002-0.5 mg/ml. Scheme of grund-immunization - 1-3 injections of antigen in the dose 3-15 ml with interval for 14-30 days, the main immunization cycle - on 45-60 day involving 10-15 injections with interval for 5-15 days and in the antigen dose 5-70 ml. Then producers are immunized once per a month with the dose 20-70 ml, and once per 6 month the shortened cycle of immunization is carried out involving 2-4 injections of antigen with interval for 5-15 days in the dose 20-70 ml. Method provides preparing heterologous antirabies serum of high specific activity.

EFFECT: improved preparing method, improved and valuable properties of serum.

12 cl, 1 tbl, 4 ex