FIELD: biotechnology. SUBSTANCE: crayfishes Gammarus were used as parental raw which were treated with diluted aqueous sodium hydroxide solution by two stages. At the first stage 0.1N aqueous sodium hydroxide solution is used and at the second stage 3 wt.-% aqueous sodium hydroxide solution is used. Then raw is treated with diluted solution of hydrochloric acid followed by washing. Obtained chitosan is subjected for deacetylation with 50 wt. -% sodium hydroxide solution at 120-130 C for 1-2 hr. EFFECT: improved method of chitosan preparing.
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