METHOD OF PREPARING ERYTHROCYTE DIAGNOSTICUM FOR PULLOROSIS-TYPHUS DIAGNOSIS IN POULTRY Russian patent published in 1996 - IPC

FIELD: veterinary microbiology. SUBSTANCE: method involves growing bacterial mass of avian pullorosis-typhus pathogen and O-antigen fraction is isolated by hydrolysis in 0.1% KOH solution at 92-94 C for 110-120 min. Sensitization of ram erythrocytes is carried out for 4 h at 45-50 C or in the process of formalinization. Sensitization is carried out at concentration of O-antigen fraction 1-1.2 mg per 1.0 cm³ 19-20% suspension of formanilized erythrocytes. To prepare erythrocyte diagnosticum sensitization can be carried out in the process of human erythrocyte formalinization after 3-3.5 h from its beginning at 45-48 C for 4 h and then at 41-42 C for 10-12 h. EFFECT: improved method of diagnosticum preparing. 2 cl, 5 tbl