ECACC N 90110707, F(AB)-FRAGMENT OF MONOCLONAL ANTIBODY PRODUCED BY THE STRAIN OF HYBRID CULTURED MURINE CELLS MUS MUSCULUS L. ECACC N 90110707, F(AB)2-FRAGMENT OF MONOCLONAL ANTIBODY PRODUCED BY THE STRAIN OF HYBRID CULTURED MURINE CELLS MUS MUSCULUS L. ECACC N 90110707, STRAIN OF HYBRID CULTURED MURINE CELLS MUS MUSCULUS L PRODUCING MONOCLONAL ANTIBODY AND METHOD OF PREPARING OF THE STRAIN OF HYBRID CULTURED MURINE CELLS MUS MUSCULUS L. ECACC N 9011070. FIELD: biotechnology, immunology. SUBSTANCE: invention relates to immunological assay of human tumor necrosis alpha-factor (F(AB)2-TNF) in biological liquids of organism. Method involves preparing monoclonal antibody interacting with α-TNF, its fragments and the strain of hybrid cells producing this antibody. Strain is prepared by fusion of murine splenocytes immunized with human a-TNF with cells of myeloma NSO. Monoclonal antibody produced by this strain and its fragments show high affinity interaction with human a-TNF and neutralize its action under conditions in vitro. EFFECT: improved method of antibody preparing. 5 cl, 6 dwg, 10 tbl
