METHOD OF THE STAINED HYALURONIC ACID PREPARING Russian patent published in 1997 - IPC

Abstract RU 2076872 C1

FIELD: biochemistry, enzymology. SUBSTANCE: method involves dissolving hyaluronic acid in water at pH = 8.0-8.5 and 95-100 C, the successive addition of dye - ethacridine lactate (rivanol) and electrolyte - potassium chloride to the prepared solution followed by incubation of this mixture containing hyaluronic acid, ethacridine lactate and potassium chloride at ratio = 1: (0.5-0.6):(0.2-0.3), respectively, at 30-40 C for 2-4 h. For isolation of the stained hyaluronic acid cooled acetone (to 4-10 C) is used at amount 3-4 volumes per 1 volume incubation mixture. Ethanol is used for the stained hyaluronic acid washing off. Method is used for substrate preparing used for quantitative enzymes and drugs assay. EFFECT: improved method of preparing.

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RU 2 076 872 C1

Authors

Stekol'Nikov L.I.

Samojlenko I.I.

Kornilova A.A.

Dates

1997-04-10Published

1993-10-21Filed