FIELD: biochemistry, enzymology. SUBSTANCE: method involves dissolving hyaluronic acid in water at pH = 8.0-8.5 and 95-100 C, the successive addition of dye - ethacridine lactate (rivanol) and electrolyte - potassium chloride to the prepared solution followed by incubation of this mixture containing hyaluronic acid, ethacridine lactate and potassium chloride at ratio = 1: (0.5-0.6):(0.2-0.3), respectively, at 30-40 C for 2-4 h. For isolation of the stained hyaluronic acid cooled acetone (to 4-10 C) is used at amount 3-4 volumes per 1 volume incubation mixture. Ethanol is used for the stained hyaluronic acid washing off. Method is used for substrate preparing used for quantitative enzymes and drugs assay. EFFECT: improved method of preparing.
Title | Year | Author | Number |
---|---|---|---|
METHOD OF HYALURONIC ACID PREPARING | 1993 |
|
RU2074196C1 |
METHOD OF HYALURONIC ACID PREPARING | 1993 |
|
RU2074863C1 |
METHOD OF PREPARING HYALURONIC ACID SHOWING BACTERICIDAL ACTIVITY | 1993 |
|
RU2092494C1 |
METHOD OF PARASITE ABOLISHMENT CAUSING DISEASE IN AQUARIUM FISHES | 1994 |
|
RU2060655C1 |
METHOD FOR PRODUCING PREPARATION FOR ARTIFICIAL SUNBURN | 1994 |
|
RU2088206C1 |
COMPOSITION FOR BATH | 1994 |
|
RU2077317C1 |
METHOD OF PREPARING VITAMIN $$$ PREPARATION FOR ORAL USE | 1993 |
|
RU2071322C1 |
METHOD OF PREPARING THE SUBSTANCE PREVENTING NIGHT DIURESIS IN YOUNG DOMESTIC ANIMALS | 1993 |
|
RU2092159C1 |
METHOD OF AROMATIC PRINCIPLE PREPARING | 1994 |
|
RU2067841C1 |
AGENT SHOWING HYPOCHOLESTEROLEMIC EFFECT AND A METHOD OF ITS PREPARING | 1994 |
|
RU2088257C1 |
Authors
Dates
1997-04-10—Published
1993-10-21—Filed