METHOD OF HYALURONIC ACID PREPARING Russian patent published in 1997 - IPC

FIELD: biotechnology, biochemistry. SUBSTANCE: method involves bleeding and milling raw - cock and hen crests, multiple washing off the milled raw with ethanol containing 1 wt.-% chloroform, extraction with 3-3.5 volumes of water acidified up to pH = 3-4 at 90-100 C for 40-60 min, deproteinization of the filtered extract by treatment firstly with activated carbon (at 60-80 C for 1-2 h) and then with diethylaminoethylcellulose used at amount 1.0-1.5% of extract volume followed by filtration by successive passing the deproteinized extract through paper filters, boronsilicate glass filters, sterile acetylcellulose and polyvinyl chloride diaphragms at 30-40 C. EFFECT: ultrapure hyaluronic acid. 2 dwg, 1 tbl