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2 189 393

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IPC

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Application

2000106867/13, 2000-03-20

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Start date

2000-03-20

(22)

Actual filing date

2000-03-20

(45)

Published

2002-09-20

(72)

Inventor

Susloparov M.A.Bakhtina M.M.Susloparov I.M.Makhova N.M.Pljasunov I.V.Lokteva L.A.

(73)

Holder

Gosudarstvennyj Nauchnyj Tsentr Virusologii I Biotekhnologii

RECOMBINANT PLASMID DNA PU11HHV6 PROVIDING EXPRESSION OF GENE U11 FRAGMENT OF HUMAN HERPES VIRUS OF 6 TYPE ENCODING IMMUNODOMINANT MOIETY OF PROTEIN TEGUMENT P100 IN CELLS OF BACTERIUM ESCHERICHIA COLI Russian patent published in 2002 - IPC

Abstract RU 2189393 C2

FIELD: biotechnology, genetic engineering. SUBSTANCE: invention relates to a constructed in vitro recombinant plasmid DNA comprising fragment of gene U11 of human herpes virus of 6-th type (HHV-6) encoding an immunodominant moiety (3 antigenic epitopes) of protein tegument p100 from 494 to 842 amino acid. The construction provides the effective biosynthesis of polypeptide in cells E. coli as a fragment of p100 HHV-6 fused with β-galactosidase fragment with histidine motif (6 His) by C-end. The use of the recombinant protein purified by method of affinity chromatography in immunoenzyme analysis for detection of immunoglobulins in patient blood samples specific to HHV-6 shows the high level of sensitivity and specificity. The recombinant protein can be used as an antigen of HHV-6 in research works and serological testing of human herpes virus of type 6 in clinical practice. EFFECT: recombinant plasmid indicated above, improved method of analysis. 4 dwg, 3 ex

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RU 2 189 393 C2

Authors

Susloparov M.A.

Bakhtina M.M.

Susloparov I.M.

Makhova N.M.

Pljasunov I.V.

Lokteva L.A.

Dates

2002-09-20—Published

2000-03-20—Filed