RECOMBINANT PLASMID DNA PUL 44 HCMV DETERMINING EXPRESSION OF HUMAN CYTOMEGALOVIRUS GENE UL 44 FRAGMENT ENCODING IMMUNODOMINANT SITE OF DNA-BINDING PROTEIN P 52 (ICP 36) IN ESCHERICHIA COLI BACTERIUM CELLS Russian patent published in 2000 - IPC

Abstract RU 2151800 C1

FIELD: biotechnology, genetic engineering, molecular biology. SUBSTANCE: recombinant plasmid DNA has human cytomegalovirus gene UL 44 fragment that encodes immunodominant site (2 antigenic epitopes) of DNA-binding viral protein p 52 (ICP 36). Structure provides biosynthesis of polypeptide as human cytomegalovirus p 52 fragment with polyhistidine tract for affinity purification that shows antigenic properties of human cytomegalovirus. The use of obtained antigen for detection of cytomegalovirus-specific immunoglobulins M (IgM-human cytomegalovirus) in patient blood shows 98-99% of sensitivity and specificity in immunoenzyme assay. The above described plasmid structure ensures to increase synthesis of the end polypeptide by 40-40 times as compared with that in prototype. EFFECT: plasmid DNA indicated above, increased yield of polypeptide. 4 dwg, 2 ex

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RU 2 151 800 C1

Authors

Susloparov M.A.

Susloparov I.M.

Pljasunov I.V.

Dates

2000-06-27—Published

1998-07-28—Filed