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2 518 329

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C1

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IPC

C07K14/505(2006-01-01)

C12N15/16(2006-01-01)

B82B1/00(2006-01-01)

A61K9/51(2006-01-01)

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Application

2012153074/10, 2012-12-07

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Start date

2012-12-07

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Actual filing date

2012-12-07

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Published

2014-06-10

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Inventor

Nechaeva Elena AvgustovnaVaraksin Nikolaj Anatol'EvichRjabicheva Tat'Jana Gennad'EvnaRadaeva Irina FedorovnaKolesova Marina Evgen'EvnaKhripko Ol'Ga PavlovnaSerebrova Varvara Sergeevna

(73)

Holder

Federal'Noe Bjudzhetnoe Uchrezhdenie Nauki Nauchnyj Tsentr Virusologii I Biotekhnologii Gnts Vb

METHOD OF PRODUCTION OF SUBSTANCE RECOMBINANT HUMAN ERYTHROPOIETIN AND NANOCAPSULAR FORM OF RECOMBINANT HUMAN ERYTHROPOIETIN WITH USE OF SUBSTANCE OBTAINED BY THIS METHOD Russian patent published in 2014 - IPC C07K14/505 C12N15/16 B82B1/00 A61K9/51 

Abstract RU 2518329 C1

FIELD: biotechnology.

SUBSTANCE: method comprises successive culturing in the growth and storage media where the growth medium comprises 5.0 wt % cattle serum and nutrient medium Igla MEM, and the storage medium comprises 49.0-80.0 mg/L proline, 40.0-60.0 mg/L glycine, and a mixture of equal parts of DMEM and F-12 media. Based on thus obtained substance of recombinant erythropoietin its nanocapsular form is prepared.

EFFECT: invention enables to obtain a substance of erythropoietin with increased specific activity and free from extraneous protein impurities and suitable for nanocapsulation, to simplify the technology of its production.

3 cl, 5 dwg, 1 tbl, 12 ex

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RU 2 518 329 C1

Authors

Nechaeva Elena Avgustovna

Varaksin Nikolaj Anatol'Evich

Rjabicheva Tat'Jana Gennad'Evna

Radaeva Irina Fedorovna

Kolesova Marina Evgen'Evna

Khripko Ol'Ga Pavlovna

Serebrova Varvara Sergeevna

Dates

2014-06-10—Published

2012-12-07—Filed

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