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Application

97103807/13, 1997-03-12

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Actual filing date

1997-03-12

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Published

1998-11-27

(72)

Inventor

Lebedev L.R.Andreeva I.S.Pustoshilova N.M.

(73)

Holder

Gosudarstvennyj Nauchnyj Tsentr Virusologii I Biotekhnologii

METHOD OF PRODUCING RECOMBINANT BACTERIUM ESCHERICHIA COLI BIOMASS ENRICHED WITH POLYPEPTIDE ELICITING HUMAN LYMPHOTOXIN PROPERTY Russian patent published in 1998 - IPC

Abstract RU 2122580 C1

FIELD: microbiology, genetic engineering, molecular biology. SUBSTANCE: method involves transformation of the strain E.coli SG 200-50 with recombinant plasmid pLT21 with DNA fragment encoding human lymphotoxin. Cell are cultured, primary transformants are selected on medium with ampicillin. For amplification with plasmid DNA the repeated sowing of selected clones is carried out on nutrient medium containing, except for ampicillin, chloramphenicol at concentration 150 mcg/ml. Flasks are sown with liquid (+ ampicillin) medium obtained by washing out colonies grown at preliminary stage. Biomass is gathered at the end of logarithmic growth phase of culture. EFFECT: increased content of human recombinant lymphotoxin in biomass. 3 tbl

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RU 2 122 580 C1

Authors

Lebedev L.R.

Andreeva I.S.

Pustoshilova N.M.

Dates

1998-11-27—Published

1997-03-12—Filed

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