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2 084 869

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C1

(51)

IPC

(21) (22)

Application

94023438/25, 1994-06-27

(22)

Actual filing date

1994-06-27

(45)

Published

1997-07-20

(72)

Inventor

Selivanov E.A.Khmylova G.A.Beljaeva I.S.Slepneva L.V.Sidorova N.D.

(73)

Holder

Rossijskij Nii Gematologii I Transfuziologii

METHOD OF QUANTITATIVE DETERMINATION OF CYTOCHROME C IN PREPARATIONS CONTAINING COLLAGEN Russian patent published in 1997 - IPC

Abstract RU 2084869 C1

FIELD: analytical chemistry. SUBSTANCE: proposed method is related to spectrophotometric processes of quantitative determination of cytochrome C in preparations containing collagen. Method includes solution of sample, optical densities of solution are measured at 400 and 490 nm. Difference of optical densities is calculated and its value is used to determine content of cytochrome C in preparation. Quantity of cytochrome C in liquid medicinal forms are calculated by product of degree of dilution of preparation by difference of optical densities and by extinction coefficient k equal to 0.115. Quantity of cytochrome C in solid medicinal forms is found by product of volume of solution by difference of optical densities and by extinction coefficient k equal to 0.115. EFFECT: high sensitivity and authenticity of determination. 3 cl, 2 tbl

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RU 2 084 869 C1

Authors

Selivanov E.A.

Khmylova G.A.

Beljaeva I.S.

Slepneva L.V.

Sidorova N.D.

Dates

1997-07-20—Published

1994-06-27—Filed

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