FIELD: microbiological industry, biotechnology. SUBSTANCE: cultural fluid obtained by culturing micromycetes Cercaspora sp. is filtered through coarse calico fabric and then through diaphragm filters "Sartorius" (pore diameter is 0.8 mcm). Obtained filtrate is passed through column filled with high-porous nonpolar resin of nonionic type that is copolymer of styrene with divinylbenzene. Then abscisic acid is eluted with acetone aqueous solution at gradient 10 at concentration range 20-50%. Method provides 70% extraction of abscisic acid from cultural fluid. EFFECT: improved method of isolation, increased yield.
Title | Year | Author | Number |
---|---|---|---|
METHOD OF ABSCISIC ACID PRODUCING | 1992 |
|
RU2085077C1 |
STRAIN OF MICROMYCETES CERCOSPORA SP - A PRODUCER OF PHYTOHORMONE, ABSCISIC ACID | 1992 |
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METHOD OF PRODUCING ANTIBIOTIC ACTINOPLACIN, STRAIN STREPTOMYCES (KITASATOA) SPECIES 834 VNIISKHM D-484 AS PRODUCER OF ANTIBIOTIC ACTINOPLACIN | 1997 |
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STRAIN OF BACTERIUM ARTHROBACTER GLOBIFORMIS - A PRODUCER OF COPROPORPHYRIN-III AND A METHOD OF COPROPORPHYRIN-III PREPARING | 1993 |
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METHOD FOR RECOVERING GIBBERELLIC ACID FROM CULTURE LIQUORS | 0 |
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SU1080747A3 |
METHOD OF ISOLATION OF CORTICOSTEROIDS FROM CULTURAL FLUID | 1992 |
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Authors
Dates
1997-07-27—Published
1992-09-16—Filed