METHOD OF ISOLATION OF ABSCISIC ACID FROM CULTURAL FLUID Russian patent published in 1997 - IPC

Abstract RU 2085588 C1

FIELD: microbiological industry, biotechnology. SUBSTANCE: cultural fluid obtained by culturing micromycetes Cercaspora sp. is filtered through coarse calico fabric and then through diaphragm filters "Sartorius" (pore diameter is 0.8 mcm). Obtained filtrate is passed through column filled with high-porous nonpolar resin of nonionic type that is copolymer of styrene with divinylbenzene. Then abscisic acid is eluted with acetone aqueous solution at gradient 10 at concentration range 20-50%. Method provides 70% extraction of abscisic acid from cultural fluid. EFFECT: improved method of isolation, increased yield.

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RU 2 085 588 C1

Authors

Muromtsev G.S.

Krasnopol'Skaja L.M.

Kobrina N.S.

Makeeva A.P.

Dates

1997-07-27Published

1992-09-16Filed