FIELD: biotechnology, microbiological industry, analytical biochemistry. SUBSTANCE: rate of L-proline oxidation with free or immobilized cells Pseudomonas species-22 (VKPM B-4877) depends linearly on concentration of L-proline at the range 0.01-0.1 mM. The used strain Pseudomonas species-22 (VKPM B-4877) does not show pathogenicity for human and does not form spores. Time for one analysis is 5 min. Method does not require periodic biomass growing, immobilized cells can be used repeatedly. Invention can be used in microbiological industry, wine-making industry, clinical and laboratory investigations. EFFECT: high selectivity, simplified method of analysis. 2 dwg, 3 tbl
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