METHOD OF REGENERATION OF VIRUS MEASLES VACCINE STRAIN Russian patent published in 1997 - IPC

FIELD: biotechnology, vaccines. SUBSTANCE: method involves infection of the tissue culture with the parent virus followed by incubation at regime of periodical virus-containing fluid removal. In 0.5-1.5 h after infection nonadsorbed virus is transferred to new tissue culture that is incubated under the same conditions and removals of virus-containing fluid from each culture were combined. At high infectious activity of the last infected culture in 0.5-1.5 h after its incubation onset nonadsorbed virus can be transferred to the next tissue culture and removals obtained from the latter were combined with population of the regenerated strain. Method is used for live antimeasles vaccine preparing. EFFECT: improved method of measles vaccine strain regeneration. 2 cl, 3 tbl