METHOD OF IMMUNOENZYMATIC DETECTION OF OPPORTUNISTIC INFECTION PHASES Russian patent published in 1999 - IPC

FIELD: medicine. SUBSTANCE: invention relates to a method of diagnosis of infection diseases caused by opportunistic bacteria. Method is based on assay of an antibody level in patient native sera using the complement reaction binding. Method involves the comparison of an antibody level in native sera with that in sera treated with cysteine suspension and sera treated with Staphylococcus aureus suspension. The primary acute phase of disease is diagnosed at high level of antibodies in native sera, at abrupt decrease of their level after treatment with cysteine and high level after interaction with staphylococcus. The exacerbation of chronic persisting phase of disease is diagnosed at moderate level of antibodies in native sera, their moderate level after treatment with staphylococcus and low level or complete destruction of antibodies after treatment with cysteine. The chronic persisting phase of disease is diagnosed at antibody absence in native sera, their high level after treatment with cysteine and absence after interaction with staphylococcus. In the process of diagnosis antibody level is considered to be high if an extinction value is decreased in an experiment by above 50%, moderate - by 50-25% and low - by less 25% as compared with control. Method ensures to differentiate phases of an opportunistic infection distinctly. EFFECT: improved method of detection. 2 ex