METHOD FOR DIAGNOSING T-CELLULAR LYMPHOMAE OF SKIN Russian patent published in 1998 - IPC

FIELD: medicine; dermatology; hematology. SUBSTANCE: method comprises several erythrodermic alternatives. Biological material sample taken from patient is subjected to cytologic analysis. Novelty of the method resides in its features: biological material sample is constituted by blood smear which is fixed, treated with ribonuclease, colored with gallocyanin-chrome alums, followed by determining interphase chromatin of lymphocytes in microdensitometer. Parametric values are used to calculate characteristic parameter DF using formula DF = -0.14379 * OD4 + 0.00003 * 10D2 + 10,4952 * FORM_P + 0.0001 * AREA - 11,1241, where OD4 is optical density of euchromatic, FORM_P is nucleus form-factor, 10D2 is integral optical density of heterchromatin, and AREA is nucleus surface area. If parameter DF has negative value, skin T-cellular lymphoma case is diagnosed. EFFECT: higher efficiency.