METHOD OF PRODUCTION OF CYTOCHROME C Russian patent published in 2014 - IPC C12P21/00 C07K14/80 

FIELD: biotechnology.

SUBSTANCE: method comprises degreasing of hearts of pigs, cattle or horses. The prepared raw material is ground to obtain minced meat, then extracted with 2.5% trichloroacetic acid solution at the temperature of 10-15°C and pH of 3.9-4.2. The extract is separated and its sorption is carried out on the cation exchanger Nuvia™ S, equilibrated with distilled water. After completion of sorption the cation exchanger Nuvia™ S is washed with distilled water and ammonia-ammonium buffer solution. Then cytochrome C is eluted with from the cation exchanger Nuvia™ S with ammonium sulphate solution followed by its purification by diafiltration until complete removal of sulphate ammonium ions and concentration of cytochrome C by ultrafiltration.

EFFECT: invention enables to increase the yield of product, increase its purity and reduce the technological process.

3 cl, 1 tbl, 3 ex