METHOD FOR PRODUCING CYTOCHROME C Russian patent published in 2011 - IPC A61K35/34 C12P21/00 C07K14/80 

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmacology, namely a method for producing cytochrome C. The method for producing cytochrome C involving degreasing of pig, cattle and horse hearts, removal of ligaments and vessels, grinding and extraction in a trichloracetic acid solution under certain conditions, separation of the extract from stuffing, sorption of the filtered extract at extract pH in the UNOsphere™ S cation exchanger balanced with a starter buffer solution at pH 3.9-4.2, after completion of the sorption process, the cation exchanger is washed with the starter buffer solution; ballast proteins are removed from the UNOsphere™ S cation exchanger by a tris solution, and it is followed with elution of cytochrome C from the UNOsphere™ S cation exchanger with an ammonium sulphate solution, concentration and dialysis purification to remove ammonium sulphate ions completely.

EFFECT: method allows increasing product yield, providing higher purity and reducing production process.

4 cl, 1 tbl, 5 ex