FIELD: biotechnology. SUBSTANCE: analog of hirudine that possesses anticoagulation activity exceeding that of HVI is obtained by transformation of E. coli strain with recombinant vector of secretion which contains DNA fragment coding target polypeptide, and also the sequence of starting point of replication obtained from pUC plasmid. Cultivation of mentioned strain is conducted and analog of hirudine is extracted from cells or culture medium. EFFECT: higher efficiency. 11 dwg, 9 tbl
| Title |
Year |
Author |
Number |
| SECRETION VECTOR FOR PREPARING HIRUDINE HV1 (VARIANTS), RECOMBINANT STRAIN ESHERICHIA COLI - A PRODUCER OF HIRUDINE HV1 AND A METHOD OF ITS PREPARING |
1995 |
- Satoru Misava
- Khitosi Matsuda
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- Khidejuki Furuja
|
RU2130071C1 |
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1991 |
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|
RU2118365C1 |
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0 |
- Zherar Luazon
- Iv Lemuan
- Natali Laba
- Alen Ballan
|
SU1776276A3 |
| VECTOR PMT440 AT POSITIVE SELECTION FOR FOREIGN DNA FRAGMENTS CLONING |
1996 |
|
RU2105064C1 |
| Leu-HIRUDINE PRECURSOR AND METHOD FOR PREPARING Leu-HIRUDINE |
2000 |
- Khabermann Paul'
- Bender Rudol'F
|
RU2261867C2 |
| RECOMBINANT PLASMID DNA pHIG05, CODING HYBRID PROTEIN WITH HUMAN PROINSULIN Glargine, CELL Escherichia coli, TRANSFORMED BY RECOMBINANT PLASMID DNA PHIG05, AND STRAIN OF BACTERIA Escherichia coli JM109/pHIG05-PRODUCENT OF HYBRID PROTEIN WITH HUMAN PROINSULIN Glargine |
2013 |
- Shmatchenko Vadim Vasil'Evich
- Sadgjan Armen Sergeevich
|
RU2515115C1 |
| RECOMBINANT PLASMID DNA pHILP07 ENCODING HYBRID PROTEIN WITH HUMAN PROINSULIN Lispro, CELL Escherichia coli TRANSFORMED BY RECOMBINANT PLASMID DNA pHILP07, AND STRAIN OF BACTERIA Escherichia coli JM109/pHILP07 - PRODUCER OF HYBRID PROTEIN WITH HUMAN PROINSULIN Lispro |
2013 |
- Shmatchenko Vadim Vasil'Evich
|
RU2514480C1 |
| RECOMBINANT PLASMID DNA pHIASP03, ENCODING HYBRID PROTEIN WITH HUMAN PROINSULIN Aspart, CELL Escherichia coli TRANSFORMED BY RECOMBINANT PLASMID DNA pHIAsp03, AND STRAIN OF BACTERIA Escherichia coli JM109/pHIAsp03 - PRODUCER OF HYBRID PROTEIN WITH HUMAN PROINSULIN Aspart |
2013 |
- Shmatchenko Vadim Vasil'Evich
- Sadgjan Armen Sergeevich
|
RU2514578C1 |
| RECOMBINANT PLASMID DNA pHI03, CODING HYBRID PROTEIN WITH HUMAN PROINSULIN, CELL Escherichia coli, TRANSFORMED BY RECOMBINANT PLASMID DNA pHI03, AND STRAIN OF BACTERIA Escherichia coli JM109/pHI03-PRODUCENT OF HYBRID PROTEIN-PRECURSOR OF HUMAN INSULIN |
2013 |
- Shmatchenko Vadim Vasil'Evich
|
RU2515061C1 |
| DNA FRAGMENT, RECOMBINANT POLYPEPTIDE EXHIBITING ANTITHROMBIN ACTIVITY, METHOD OF ITS PREPARING (VARIANTS), PHARMACEUTICAL COMPOSITION, RECOMBINANT VECTOR (VARIANTS) |
1992 |
- Paolo Sarmentos
- Filipp De Taksis Dju Poet
- Dzhampaolo Nitti
- Ehmanuehla Skacheri
|
RU2131462C1 |
