FIELD: biotechnology, microbiology, medicine.
SUBSTANCE: method involves selection of signal sequence suitable for the effective expression of Leu-hirudine in E. coli cells by the polymerase chain reaction-screening method. Method involves construction of a protein as a precursor of hirudine based on the selected signal sequence of surface membrane protein from Serratia marcescens, oprF protein from Pseudomonas fluorescens or fumarate reductase from Shewanella putrifaciens by joining the Leu-hirudine amino acid sequence with C-end of selected signal sequence. Prepared precursor of Leu-hirudine is used in a method for preparing Leu-hirudine. Invention provides preparing Leu-hirudine by the direct secretion in E. coli cells with the high yield. Invention can be used in preparing the hirudine precursor.
EFFECT: improved preparing method.
4 cl, 1 dwg, 2 tbl, 12 ex
| Title |
Year |
Author |
Number |
| MODIFIED SIGNAL PEPTIDE OF ESCHERICHIA COLI ENTEROTOXIN-II AND MICROORGANISM EXPRESSING FUSION PROTEIN OF MENTIONED PEPTIDE WITH HETEROLOGICAL PROTEIN |
1999 |
- Kvon Se Chang
- Dzung Sung Joub
- Skhin Khoon
- Choj Dzaj Do
- Choj Ki Doo
- Li Gvan Sun
|
RU2198179C2 |
| METHOD TO PRODUCE RECOMBINANT ANTI-ANGIOGENIC POLYPEPTIDE |
2012 |
- Glybochko Petr Vital'Evich
- Svistunov Andrej Alekseevich
- Nikolenko Vladimir Nikolaevich
- Lutsenko Sergej Viktorovich
- Fel'Dman Natalija Borisovna
- Solov'Ev Andrej Ivanovich
|
RU2488635C1 |
| METHOD FOR PRODUCING PROTEIN |
2006 |
- Itaja Khirosi
- Kikuti Josimi
- Date Masae
|
RU2435863C2 |
| HEMOPOIETIC PROTEIN (VARIANTS), DNA SEQUENCE, METHOD FOR PREPARING HEMOPOIETIC PROTEIN, METHOD FOR SELECTIVE EXPANSION OF HEMOPOIETIC CELLS, METHOD FOR PRODUCING DENDRITIC CELLS, METHOD FOR STIMULATING FOR PRODUCING HEMOPOIETIC CELLS IN PATIENT, PHARMACEUTICAL COMPOSITION, METHOD FOR TREATMENT |
1997 |
- Mak Verter Charl'Z A.
- Feng Jikving
- Mak Kern Dzhon P.
- Sammers Nina L.
- Stehjten Nikolas R.
- Striter Filip R.
- Minnerli Dzhon K.
- Minster Nehnsi I.
- Vul'F S'Juzn L.
|
RU2245887C2 |
| RECOMBINANT PLASMID DNA pHIG05, CODING HYBRID PROTEIN WITH HUMAN PROINSULIN Glargine, CELL Escherichia coli, TRANSFORMED BY RECOMBINANT PLASMID DNA PHIG05, AND STRAIN OF BACTERIA Escherichia coli JM109/pHIG05-PRODUCENT OF HYBRID PROTEIN WITH HUMAN PROINSULIN Glargine |
2013 |
- Shmatchenko Vadim Vasil'Evich
- Sadgjan Armen Sergeevich
|
RU2515115C1 |
| METHOD FOR SECRETORY PROTEIN PRODUCTION |
2002 |
- Kikuti Josimi
- Date Masajo
- Umezava Jukiko
- Jokojama Kejiti
- Kheima Kharuo
- Matsui Khirosi
|
RU2264463C2 |
| SECRETION VECTOR FOR PREPARING HIRUDINE HV1 (VARIANTS), RECOMBINANT STRAIN ESHERICHIA COLI - A PRODUCER OF HIRUDINE HV1 AND A METHOD OF ITS PREPARING |
1995 |
- Satoru Misava
- Khitosi Matsuda
- Esifumi Inoue
- Khidejuki Furuja
|
RU2130071C1 |
| METHOD OF POLYPEPTIDE PRODUCING IN YEAST |
1995 |
- K'El'Dsen Tomas Berglum
- Vad Knud
|
RU2167939C2 |
| RECOMBINANT PLASMID DNA pHIASP03, ENCODING HYBRID PROTEIN WITH HUMAN PROINSULIN Aspart, CELL Escherichia coli TRANSFORMED BY RECOMBINANT PLASMID DNA pHIAsp03, AND STRAIN OF BACTERIA Escherichia coli JM109/pHIAsp03 - PRODUCER OF HYBRID PROTEIN WITH HUMAN PROINSULIN Aspart |
2013 |
- Shmatchenko Vadim Vasil'Evich
- Sadgjan Armen Sergeevich
|
RU2514578C1 |
| PLASMID FOR EXPRESSION IN BACTERIA CELLS OF GENUS Escherichia OF PRECURSOR OF RECOMBINANT FRAGMENT OF HUMAN TISSUE PLASMINOGEN ACTIVATOR (TPA) (RETEPLASE), BACTERIA BELONGING TO GENUS Escherichia, - PRODUCER OF PRECURSOR OF RECOMBINANT FRAGMENT OF HUMAN TPA (RETEPLASE) OR [-1] METHIONYL - FRAGMENT OF HUMAN TPA (RETEPLASE), PRECURSOR OF RECOMBINANT FRAGMENT OF HUMAN TPA (RETEPLASE), METHOD OF PRODUCTION OF PRECURSOR OF RECOMBINANT FRAGMENT OF HUMAN TPA (RETEPLASE) |
2011 |
- Orlova Nadezhda Aleksandrovna
- Vorob'Ev Ivan Ivanovich
|
RU2495933C2 |
