FIELD: biotechnology, dairy, medicinal and pharmaceutical industries. SUBSTANCE: cow milk is used for isolation of angiogenine. Raw is purified by centrifugation, proteins are sorbed on ion-exchange resin and protein fractions are eluted at wavelength 280 nm at optical value above 0.1. Then dialysis and ion-exchange chromatography are carried out. Fraction containing angiogenine are identified, combined and hydrophobic chromatography is carried out. Then eluates are desalted and concentrated and obtained angiogenine is stabilized by ammonium sulfate. EFFECT: simple, effective and cheap method of isolation. 2 cl
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF PREPARING BIOLOGICALLY ACTIVE SUPPLEMENT "MILKANG" AND BIOLOGICALLY ACTIVE "MILKANG" PREPARED BY SAID METHOD | 2000 |
|
RU2183935C2 |
| METHOD OF ISOLATING ANIGIOGENIN-RICH PROTEIN FRACTION | 2000 |
|
RU2175195C1 |
| METHOD FOR PRODUCTION OF BIOLOGICALLY ACTIVE SUPPLEMENT (BAS) FROM LAW MOLECULAR MILK CATION PROTEINS AND OBTAINED BAS | 2006 |
|
RU2318406C1 |
| DIETARY SUPPLEMENT "MOBELIS" AND ITS PRODUCTION METHOD | 2008 |
|
RU2366294C1 |
| METHOD OF QUANTITATIVELY DETERMINING ANGIOGENIN IN BIOLOGICAL SAMPLE | 1996 |
|
RU2110066C1 |
| METHOD FOR PRODUCTION OF "L-PFI" DIETARY SUPPLEMENT FROM SECONDARY MILK STOCK AND "L-PFI" DIETARY SUPPLEMENT PRODUCED BY THIS METHOD | 2009 |
|
RU2400106C1 |
| METHOD FOR PREPARING BIOLOGICALLY ACTIVE PROTEIN CONCENTRATE ENRICHED WITH PANCREATIC RIBONUCLEASE A, ANGIOGENIN AND LYSOZYME FROM DAIRY ULTRAFILTRATE | 2001 |
|
RU2204262C2 |
| METHOD FOR QUANTITATIVELY DETERMINING ANGIOGENINE IN MILK MATERIAL | 1998 |
|
RU2133464C1 |
| STERILIZED ANGIOGENINE-ENRICHED MILK | 2000 |
|
RU2182791C2 |
| METHOD OF COLLASE PURIFICATION | 1996 |
|
RU2121503C1 |
