FIELD: biotechnology. SUBSTANCE: curculin B showing taste modifying agent activity and identified amino acid sequence is obtained by transformation of strains of Escherichia coli with expression vector containing DNA fragment that encodes curculin B. Nucleotide sequence of DNA fragment is identified. Transformed strains are cultured and curculin B is isolated. EFFECT: improved method of preparing. 8 dwg, 2 tbl, 6 ex
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Year |
Author |
Number |
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1992 |
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- Takeaki Akabane[Jp]
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